LDHA Knockdown 293T Cell Lysate, Heterozygous (RM02019)

Genome sequence analysis of PCR products from parental (WT) and LDHA Knockdown (KD) 293T cells, using sanger sequencing.

All(1)|

货号: RM02019

详细信息

靶点

LDHA

细胞系

293T

突变描述

LDHA Knockdown 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:exon2 was deleted
Allele-2:WT

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

基因ID

3939

Swiss Prot

P00338

别名

GSD11; HEL-S-133P; LDHM; PIG19

研究领域