STK4 Knockout HeLa Cell Lysate, Homozygous (RM02041)

Genome sequence analysis of PCR products from parental (WT) and STK4 knockout (KO) HeLa cells, using sanger sequencing.

Western blot analysis of extracts from parental (Control) and STK4 knockout (KO) HeLa cells, using STK4 antibody (A18100) at 1:1000 dilution.

All(2)|

货号: RM02041

详细信息

靶点

STK4

细胞系

HeLa

突变描述

STK4 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:46bp deletion in exon3
Allele-2:1bp insertion in exon3

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]

基因ID

6789

Swiss Prot

Q13043

别名

KRS2; MST1; YSK3

研究领域