MYC Knockdown HCT116 Cell Lysate, Heterozygous (RM02247)

Genome sequence analysis of PCR products from parental (WT) and MYC Knockdown (KD) HCT116 cells, using sanger sequencing.

All(1)|

货号: RM02247

详细信息

靶点

MYC

细胞系

HCT116

突变描述

MYC Knockdown HCT116 Cell Line is engineered from HCT116 cell line with Gene-Editing technology.
Allele-1:177bp deletion in exon2
Allele-2:199bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

文献引用 (1)

折叠内容

Publishing research using RM02247? Please let us know so that we can cite the reference in this datasheet.

背景信息

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]

基因ID

4609

Swiss Prot

P01106

别名

MRTL; MYCC; bHLHe39; c-Myc

研究领域