PRKAA1 Knockout HeLa Cell Lysate, Homozygous (RM02255)

Genome sequence analysis of PCR products from parental (WT) and PRKAA1 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02255

详细信息

靶点

PRKAA1

细胞系

HeLa

突变描述

PRKAA1 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:1bp deletion in exon2
Allele-2:2bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

基因ID

5562

Swiss Prot

Q13131

别名

AMPK; AMPKa1

研究领域