产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

SOD2 Knockdown 293T Cell Lysate, Heterozygous (RM02267)

说明书 收藏

Genome sequence analysis of PCR products from parental (WT) and SOD2 Knockdown (KD) 293T cells, using sanger sequencing.

All(1)|
货号: RM02267
促销价：   ￥4700
抗体定制
服务咨询 |
扫码下单
享受积分

详细信息

靶点
SOD2
细胞系
293T
突变描述
SOD2 Knockdown 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:98bp deletion in exon2
Allele-2:97bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]

基因ID
6648
Swiss Prot
P04179
别名
IPO-B; IPOB; MNSOD; MVCD6; Mn-SOD
研究领域

最新浏览的产品