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METTL3 Knockdown 293T Cell Lysate, Heterozygous (RM02306)

Genome sequence analysis of PCR products from parental (WT) and METTL3 Knockdown (KD) 293T cells, using sanger sequencing.

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货号: RM02306
促销价:   ¥4700
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详细信息

靶点
METTL3
细胞系
293T
突变描述
METTL3 Knockdown 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:138bp deletion in exon2
Allele-2:139bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]

基因ID
Swiss Prot
别名
IME4; M6A; MT-A70; Spo8; hMETTL3
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