突变描述
CDKL4 Knockout NIH/3T3 Cell Line is engineered from NIH/3T3 cell line with Gene-Editing technology.
Allele-1:61bp deletion in exon3
Allele-2:2bp deletion in exon3
Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
