TXNIP Knockout HuH-7 Cell Lysate, Homozygous (RM02405)

Genome sequence analysis of PCR products from parental (WT) and TXNIP knockout (KO) Huh-7 cells, using sanger sequencing.

All(1)|

货号: RM02405

详细信息

靶点

TXNIP

细胞系

HuH-7

突变描述

TXNIP Knockout HuH-7 Cell Line is engineered from HuH-7 cell line with Gene-Editing technology.
Allele-1:76bp deletion in exon2
Allele-2:76bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

基因ID

10628

Swiss Prot

Q9H3M7

别名

ARRDC6; EST01027; HHCPA78; THIF; VDUP1

研究领域