产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

CXCL10 Knockout HeLa Cell Lysate, Homozygous (RM02563)

Genome sequence analysis of PCR products from parental (WT) and CXCL10 knockout (KO) HeLa cells, using sanger sequencing.

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货号: RM02563
促销价:   ¥4700
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详细信息

靶点
CXCL10
细胞系
HeLa
突变描述
CXCL10 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:80bp deletion in exon2;Allele-2:80bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Sep 2014]

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