MGAT1 with GNT1 Knockout 293F Cell Lysate, Homozygous (RM02769)

Genome sequence analysis of PCR products from parental (WT) and MGAT1 with GNT1 knockout (KO) 293F cells, using sanger sequencing.

All(1)|

货号: RM02769

详细信息

靶点

MGAT1,GNT1

细胞系

293F

突变描述

MGAT1,GNT1 Knockout cell line is engineered from 293F cell line with Gene-Editing Technology.
Allele-1:exon3 was deleted，Allele-2:85bp deletion in exon3(GNT1)
Allele-1:53bp deletion in exon1,Allele-2:52bp deletion in exon1(MGAT1)

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

基因ID

4245

Swiss Prot

P26572

别名

GnTI; MGAT; GLCT1; GLYT1; GNT-1; GNT-I; GLCNAC-TI; MGAT1