MGAT1 with GNT1 Knockout 293F Cell Lysate, Homozygous (RM02769)

MGAT1,GNT1 Knockout cell line is engineered from 293F cell line with Gene-Editing Technology.
Allele-1:exon3 was deleted，Allele-2:85bp deletion in exon3(GNT1)
Allele-1:53bp deletion in exon1,Allele-2:52bp deletion in exon1(MGAT1)

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.