KRT8 Knockout HeLa Cell Lysate, Homozygous (RM02787)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and KRT8 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02787

详细信息

靶点

KRT8

细胞系

HeLa

突变描述

KRT8 Knockout cell line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:47bp deletion in exon2
Allele-2:3bp insertion and 26bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

基因ID

3856

Swiss Prot

P05787

别名

K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; Cytokeratin 8 (KRT8)

研究领域