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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
Fromthe comparison of the standard curve, it is found that ABclonal has highsensitivity.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Mouse TNF-α Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4ug/mL | Rabbit anti-Mouse TNF-α(CAP)(Cat. No.RM17776) |
| ELISA Detection | 0.04-0.16ug/mL | Rabbit anti-Mouse TNF-α(DET)(Cat. No.RM17777) |
| Standard | 2.3-150pg/mL | Recombinant Mouse TNF-α Protein |
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity).Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance
