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GDF3 Knockout 293T Cell Lysate, Homozygous (RM50006)

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Genome sequence analysis of PCR products from parental (WT) and GDF3 knockout (KO) 293T cells, using sanger sequencing.

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货号: RM50006
促销价：   ￥4700
货    期：现货产品
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详细信息

靶点
GDF3
细胞系
293T
突变描述
GDF3 Knockout cell line is engineered from 293T cell line with Gene-Editing Technology.
Allele-1:82bp deletion in exon1
Allele-2:82bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein plays a role ocular and skeletal development. Mutations in this gene are associated with microphthalmia, coloboma, and skeletal abnormalities in human patients.

基因ID
9573
Swiss Prot
Q9NR23
别名
KFS3; MCOP7; MCOPCB6; GDF3
研究领域
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