HOPX Knockout 293T Cell Lysate, Homozygous (RM50013)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and HOPX knockout (KO) 293T cells, using sanger sequencing.

All(1)|

货号: RM50013

详细信息

靶点

HOPX

细胞系

293T

突变描述

HOPX Knockout cell line is engineered from 293T cell line with Gene-Editing Technology.
Allele-1:95bp deletion in exon1
Allele-2:95bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene.

基因ID

84525

Swiss Prot

Q9BPY8

别名

HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31; HOPX

研究领域