YWHAB Knockout HeLa Cell Lysate, Homozygous (RM50036)

Genome sequence analysis of PCR products from parental (WT) and YWHAB knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM50036

详细信息

靶点

YWHAB

细胞系

HeLa

突变描述

YWHAB Knockout cell line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:127bp deletion in exon1
Allele-2:127bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

基因ID

7529

Swiss Prot

P31946

别名

HS1; GW128; YWHAA; KCIP-1; HEL-S-1; 14-3-3 alpha/beta

研究领域