CDKN3 Knockout 293T Cell Lysate, Homozygous (RM50102)

Genome sequence analysis of PCR products from parental (WT) and CDKN3 knockout (KO) 293T cells, using sanger sequencing.

All(1)|

货号: RM50102

详细信息

靶点

CDKN3

细胞系

293T

突变描述

CDKN3 Knockout cell line is engineered from 293T cell line with Gene-Editing Technology.
Allele-1:exon4 was deleted
Allele-2:exon4 was deleted

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

基因ID

1033

Swiss Prot

Q16667

别名

KAP; CDI1; CIP2; KAP1; CDKN3

研究领域