CP Knockout HeLa Cell Lysate, Homozygous (RM50149)

说明书联系我们收藏

Genome sequence analysis of PCR products from parental (WT) and CP knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM50149

详细信息

靶点

CP

细胞系

HeLa

突变描述

CP Knockout cell line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:161bp deletion in exon2
Allele-2:161bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.

基因ID

1356

Swiss Prot

P00450

别名

CP-2; AB073614; Ceruloplasmin

研究领域