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HDAC1 Knockdown 293F Cell Lysate, Heterozygous (RM50150)

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Genome sequence analysis of PCR products from parental (WT) and HDAC1 knockdown (KD) 293F cells, using sanger sequencing.

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货号: RM50150
促销价：   ￥4700
货    期：现货产品
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详细信息

靶点
HDAC1
细胞系
293F
突变描述
HDAC1 Knockdown cell line is engineered from 293F cell line with Gene-Editing Technology.
Allele-1:66bp deletion in exon2
Allele-2:44bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

基因ID
3065
Swiss Prot
Q13547
别名
HD1; RPD3; KDAC1; GON-10; RPD3L1; C1
研究领域
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