产品和服务 / 产品类型 / 重组蛋白 / 其他蛋白

活性验证

Recombinant Human S100-A12 Protein (RP00009)

文献引用 (1) 说明书 收藏

Recombinant Human S100-A12 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 13 kDa.

Immobilized Recombinant Human S100-A12 Protein at 2μg/mL (100 μL/well) can bind AGER with a linear range of 1.95-14.13ng/mL.

All(2)|
货号: RP00009
促销价：   ￥2000
货    期：现货产品
抗体定制服务咨询 |
扫码下单
享受积分

详细信息

种属
Human
表达宿主
E. coli
Calculated MW
11.42 kDa
Observed MW
13 kDa
标签
C-His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human S100-A12 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Glu92) of human S100A12 (Accession #NP_005612.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human S100A12 Protein at 2 μg/mL (100 μL/well) can bind AGER with a linear range of 1.95-14.13 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

客户数据及评论 (0)

折叠内容

文献引用 (1)

折叠内容

Publishing research using RP00009? Please let us know so that we can cite the reference in this datasheet.

背景信息

The protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 proteins include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.

基因ID
6283
Swiss Prot
P80511
别名
S100A12;CAAF1;CAGC;CGRP;ENRAGE;MRP-6;MRP6;p6
研究领域
>