Recombinant Human IL-36 gamma/IL-1F9 Protein (RP00035)

Recombinant Human IL36 gamma/IL-1F9 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 17 kDa.

The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-36 gamma protein. After 7 hours, 2.5 ng/mL of human IL-36 gamma can effectively activate the NF-κB signaling.

Recombinant Human IL-36G induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED₅₀ for for this effect is 101.34-405.36 ng/mL.

All(3)|

货号: RP00035

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

17.03 kDa

Observed MW

17 kDa

标签

No tag

纯度

> 92% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.

描述

Recombinant Human IL-36 gamma/IL-1F9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser18-Asp169) of human Interleukin-36 gamma (Accession #NP_062564.1 ).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-36 gamma protein. After 7 hours, 2.5 ng/mL of human IL-36 gamma can effectively activate the NF-κB signaling.
2.Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED₅₀ for for this effect is 101.34-405.36 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

The protein is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection.

基因ID

56300

Swiss Prot

Q9NZH8

别名

IL36G;IL-1F9;IL-1H1;IL-1RP2;IL1E;IL1F9;IL1H1;IL1RP2

研究领域