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活性验证

Recombinant Human AGER/RAGE Protein (RP00154)

Active Recombinant Human AGER/RAGE Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100 kDa.

The purity of Human AGER/RAGE Protein (Cat.RP00154) was greater than 95% as determined by SEC-HPLC.

Immobilized Recombinant human HMGB1 at 2 μg/mL (100 μL/well) can bind Recombinant human AGER with a linear range of 15-50 ng/mL.

Immobilized Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE, the EC50 of Human AGER/RAGE is 27.25 ng/mL.

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货号: RP00154
促销价:   ¥1000
货    期:现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
60.89 kDa
Observed MW
80-100 kDa
标签
C-hFc&His
纯度
> 90% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human AGER/RAGE Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln24-Ala344) of human AGER/RAGE (Accession #NP_001127.1) fused with an Fc, 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant human HMGB1 at 2 μg/mL (100 μL/well) can bind Recombinant human AGER with a linear range of 15-50 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE, the EC50 of Human AGER/RAGE is 27.25 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

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背景信息

Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.

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