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|描述||Active Recombinant Human TGF-beta 1(C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus.|
|纯度||> 95% by SDS-PAGE.|
|内毒素||< 0.1 EU/μg of the protein by LAL method.|
|生物活性||1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL.|
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
|制剂||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.|
|储存||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|复溶||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl|
|别名||TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; transforming growth factor beta-1;TGF-beta 1;CED;DPD1;LAP;TGFB;TGFbeta;TGF-β|
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