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| 种属 | Human |
|---|---|
| 表达宿主 | HEK293 cells |
| 描述 | Active Recombinant Human TGF-beta 1(C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus. |
| 标签 | N-His |
| 纯度 | > 95% by SDS-PAGE. |
| 内毒素 | < 0.1 EU/μg of the protein by LAL method. |
| 生物活性 | 1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL. |
| 制剂 | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 储存 | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
| 复溶 | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl |
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