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Active Recombinant Human Latent TGF-beta 1 (C33S) Protein (RP00161)

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Active Recombinant Human TGF-beta 1(C33S) Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 13&42&60kDa.

Recombinant Human TGF-beta 1 inhibits cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL, corresponding to a specific activity of 5.0×105~2.0×106 units/mg.

Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.

Recombinant Human TGF-beta 1 inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 9.75-39 ng/mL, corresponding to a specific activity of 2.6×104~1.0×105 units/mg.

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货号: RP00161
促销价：   ￥780
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Active Recombinant Human Latent TGF-beta 1 (C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus.
标签
N-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL, corresponding to a specific activity of 5.0×105~2.0×106 units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
3.Measured by its ability to inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 9.75-39 ng/mL, corresponding to a specific activity of 2.6×104~1.0×105 units/mg.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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折叠内容

Publishing research using RP00161? Please let us know so that we can cite the reference in this datasheet.

背景信息

基因ID
7040
Swiss Prot
P01137
别名
TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; transforming growth factor beta-1;TGF-beta 1;CED;DPD1;LAP;TGFB;TGFbeta;TGF-β
研究领域

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