Recombinant Human SIRP-alpha/CD172a Protein (RP00171)

Recombinant Human SIRP-alpha/CD172a Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-100 kDa.

The purity of Human SIRP alpha/CD172a Protein (Cat.RP00171) was greater than 90% as determined by SEC-HPLC.

Immobilized Recombinant Human CD47 at 1 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha with a linear range of 1-6 ng/mL.

Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(Catalog: RP00171) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (Catalog: RP01306) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).

All(4)|

货号: RP00171

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

64.10 kDa

Observed MW

80-100 kDa

标签

C-hFc&His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human SIRP-alpha/CD172a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu31-Arg370) of human SIRP alpha/CD172a (Accession #NP_542970.1) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD47 at 1 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha with a linear range of 1-6 ng/mL.
2.Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(Catalog: RP00171) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (Catalog: RP01306) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).
3.Measured by the ability of the immobilized protein to support the adhesion of the OVCAR-3 cells. The ED₅₀ for this effect is 0.53-2.12 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

The protein is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein.

基因ID

140885

Swiss Prot

P78324

别名

BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP;SIRPA;CD172A;MFR;MYD-1;P84;PTPNS1;SHPS1;SIRP

研究领域