Recombinant Human TNFSF11/RANKL/CD254 Protein (RP00183)

说明书收藏

Recombinant Human TRANCE/RANK L/TNFSF11 was determined by SDS-PAGE with Coomassie Blue, showing a band at 35 kDa.

The purity of Human TRANCE/RANK L/TNFSF11 Protein (Cat.RP00183) was greater than 90% as determined by SEC-HPLC.

Immobilized Human TNFSF11 Protein at 2 μg/mL (100 μL/well) can bind Mouse TNFRSF11B with a linear range of 0.049-13.74 ng/mL.

Measured by its ability to induce osteoclast differentiation of mouse bone marrow cells.

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED₅₀ for this effect is 10.85-43.42 ng/mL, corresponding to a specific activity of 2.30×10⁴~9.22×10⁴ units/mg.

All(5)|

货号: RP00183

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

21.32 kDa

Observed MW

35 kDa

标签

N-His

纯度

> 90% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human TNFSF11/RANKL/CD254 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly136-Asp317) of human TRANCE/RANK L/TNFSF11 (Accession #NP_143026.1) fused with a 6×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF11 Protein at 2 μg/mL (100 μL/well) can bind Mouse TNFRSF11B with a linear range of 0.049-13.74 ng/mL.
2.Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 10.85-43.42 ng/mL, corresponding to a specific activity of 2.30×104~9.22×104 units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. TNFSF11 is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy.

基因ID

8600

Swiss Prot

O14788

别名

TNFSF11;CD254;ODF;OPGL;OPTB2;RANKL; TNLG6B;TRANCE;hRANKL2;sOdf

研究领域