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活性验证

Recombinant Human IL-6 Protein (RP00201)

文献引用 (3) 评论 (2) 说明书 收藏

Recombinant Human IL-6 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 23-30kDa.

Immobilized Human IL6R at 1 μg/mL (100 μL/well) can bind Human IL-6 with a linear range of 2-15 ng/mL.

Recombinant Human IL6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 for this effect is 0.07-0.26 ng/mL, corresponding to a specific activity of 3.85 × 106~1.43 × 107 units/mg.

All(3)|
货号: RP00201

促销价：   ￥480
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
21.65 kDa
Observed MW
23-30
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human IL-6 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Val30-Met212) of human IL6 (Accession #NP_000591.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA.Immobilized Human IL6R at 1 μg/mL (100 μL/well) can bind Human IL-6 with a linear range of 2-15 ng/mL.
2.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.07-0.26 ng/mL, corresponding to a specific activity of 3.85 × 106~1.43 × 107 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

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客户数据及评论 (2)

折叠内容
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
HSPCs
Description
Recombinant TPO (50 ng/mL, Cat. RP00174), IL-3 (15 ng/mL, Cat. RP01362), IL-6 (15 ng/mL, Cat. RP00201) and IL-11 (15 ng/mL, Cat. RP00050) were used to induce HSPCs differentiation into megakaryocytes. After 6 days of induction, the proportion of cells expressed CD41/42+ reached 42.5%.
CustomerOct 232024
评分
Experiment Type
Cell-based assay
Sample
KMS-12-PE
Description
Human myeloma cell line KMS-12-PE was stimulated with 50 ng/mL IL-6 for 0, 15, 30, 60 min. WB detection showed that STAT3 phosphorylation activation was most significant when stimulated for 30min.

文献引用 (3)

折叠内容

Publishing research using RP00201? Please let us know so that we can cite the reference in this datasheet.

背景信息

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. The encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this protein is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.

基因ID
3569
Swiss Prot
P05231
别名
IL6;BSF-2;BSF2;CDF;HGF;HSF;IFN-beta-2;IFNB2;IL-6
研究领域
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