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Recombinant Human B7-H5/Gi24/VISTA Protein (RP00303)

说明书 收藏

Human B7-H5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human B7-H5 is greater than 95% as determined by SEC-HPLC

Immobilized Human B7-H5, hFc Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.0ng/mL determined by ELISA (QC Test).

Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human IGSF11, His Tag with an affinity constant of 14.44 μM as determined in SPR assay (Biacore T200).

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货号: RP00303
促销价：   ￥2880
货    期：在线咨询货期
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
44.9 kDa
Observed MW
70-80 kDa
标签
C-hFc
纯度
> 95% by SDS-PAGE；> 95% by HPLC
内毒素
< 1 EU/μg of the protein by LAL method
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
描述
Recombinant Human B7-H5/Gi24/VISTA Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe33-Ala194) of Human B7-H5/Gi24/VISTA (Accession #Q9H7M9) fused with a C-hFc tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.

基因ID
64115
Swiss Prot
Q9H7M9
别名
B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1

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