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Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein (RP00496)

文献引用 (1) 说明书 收藏

Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP00496

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详细信息

种属
Human
表达宿主
E. coli
Calculated MW
13.6 kDa
Observed MW
14 kDa
标签
No tag
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.Contact us for customized product form or formulation.
描述
Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Tyr139-Thr257) of human NT-3/Neurotrophin-3 (Accession #P20783).
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (1)

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Publishing research using RP00496? Please let us know so that we can cite the reference in this datasheet.

背景信息

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptideand a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequencesof mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survivalof specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

基因ID
4908
Swiss Prot
P20783
别名
NTF3;HDNF;NGF-2;NGF2;NT-3;NT3
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