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活性验证

Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein (RP00496)

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Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human TrkB (Catalog: RP00290) at 2μg/mL (100 μL/well) can bind Recombinant Human Neurotrophin-3/NGF-2/NT-3 with a linear range of 1.95-35.5 ng/mL.

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货号: RP00496
价 格：  1100.00
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详细信息

靶点
NTF3
细胞系
13.6 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 5.2.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
14 kDa
产品组成
Tyr139-Thr257
物种
E. coli
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Publishing research using RP00496? Please let us know so that we can cite the reference in this datasheet.

背景信息

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptideand a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequencesof mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survivalof specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

基因ID
4908
Swiss Prot
P20783
别名
NTF3;Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor