Biotinylated Recombinant Human CSF2RA/GM-CSF R alpha/CD116 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/mL determined by ELISA (QC Test).
Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
