Biotinylated Recombinant Human CD30/TNFRSF8 Protein (RP00557B)

Biotinylated CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated CD30 is greater than 95% as determined by SEC-HPLC.

Immobilized Anti-CD30 Antibody, hFc Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC₅₀ of 11.5ng/mL determined by ELISA (QC Test).

Immobilized Human CD30 Ligand, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC₅₀ of 27.1ng/mL determined by ELISA.

All(4)|

货号: RP00557B

详细信息

种属

Human

表达宿主

HEK293 cells

描述

Biotinylated Recombinant Human CD30/TNFRSF8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe19-Lys379) of Human CD30/TNFRSF8 (Accession #P28908-1) fused with a C-His&Avi tag at the C-terminus.

标签

C-His&Avi

纯度

> 95% by SDS-PAGE；> 95% by HPLC

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

储存

Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).

基因ID

943

Swiss Prot

P28908-1

别名

CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1

研究领域

Biotinylated Recombinant Human CD30/TNFRSF8 Protein (RP00557B)

产品导航

Biotinylated Recombinant Human CD30/TNFRSF8 Protein (RP00557B)

详细信息

蛋白复溶计算器

背景信息

CD30/TNFRSF8 相关产品