Recombinant Human Tissue-type plasminogen activator/TPA/PLAT Protein (RP00595)

Recombinant Human tPA/Tissue-type plasminogen activator/PLAT Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP00595

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

60.1 kDa

Observed MW

55-80 kDa

标签

C-6×His

纯度

> 95% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.2 μm filtered solution of 20mM MES,150mM NaCl,0.2mM CaCl₂, pH5.5. Contact us for customized product form or formulation.

描述

Recombinant Human Tissue-type plasminogen activator/TPA/PLAT Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ser36-Pro562) of Human Tissue-type plasminogen activator/TPA/PLAT (Accession #P00750) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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文献引用 (2)

折叠内容

Publishing research using RP00595? Please let us know so that we can cite the reference in this datasheet.

背景信息

Tissue-type plasminogen activator (PLAT) is a protein that secreted into extracellular space. PLAT contains five domains:EGF-like domain, fibronectin type-I domain, 2 kringle domains and peptidase S1 domain. It belongs to the peptidase S1family. The main function of this protein is to convert plasminogen into biologically active plasmin. As a protease, PLATplays a crucial role in regulating blood fibrinolysis, maintaining the homeostasis of extracellular matrix and in modulatingthe post-translational activation of growth factors. PLAT is found not only in the blood, where its primary function is as athrombolytic enzyme, but also in the central nervous system (CNS). It participates in a number of physiological andpathological events in the CNS, as well as the role of neuroserpin as the natural regulator of PLAT's activity in theseprocesses. Increased or decreased activity of PLAT leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, asa cytokine, PLAT plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as afibrogenic cytokine, it promotes the progression of kidney diseases.

基因ID

5327

Swiss Prot

P00750

别名

PLAT;T-PA;TPA; TPA

研究领域