Recombinant Human uPA/PLAU Protein (RP00652)

说明书收藏

Human PLAU (activated by trypsin) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human PLAU (activated by trypsin) is greater than 95% as determined by SEC-HPLC.

All(2)|

货号: RP00652

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

17.9, 29.2, 15.3 kDa

Observed MW

30-38, 17 kDa

标签

C-His

纯度

> 95% by SDS-PAGE；> 95% by HPLC

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in 1% HCOOH, 1 mM DTT (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Human uPA/PLAU Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser21-Leu431) of Human uPA/PLAU (Accession #P00749-1) fused with a C-His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Please contact us for reconstitution instructions.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

基因ID

5328

Swiss Prot

P00749-1

别名

PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD

研究领域