Recombinant Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus. It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe177) and N-Terminus fragment (Ser21-Lys155) .

" />Recombinant Human uPA/PLAU Protein-免疫检查点- ABclonal

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活性验证

Recombinant Human uPA/PLAU Protein (RP00652)

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货号: RP00652
价 格：  4000.00
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
17.9, 29.2, 15.3 kDa
Observed MW
30-38, 17 kDa
标签
C-His
纯度
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from 0.22 μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
描述

Recombinant Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus. It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe177) and N-Terminus fragment (Ser21-Lys155) .

储存
Store at -20℃ （1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/μg.
复溶
Please contact us for reconstitution instructions.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

基因ID
5328
Swiss Prot
P00749-1
别名
PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
研究领域