详细信息
制剂
Lyophilized from a 0.2 μm filtered solution of 50 mM Glycine,150 mM NaCl, pH3.5. Contact us for customized product form or formulation.
描述
Recombinant Mouse TGF-beta 1/TGFB1 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ala279-Ser390) of mouse TGF-beta 1/TGFB1 (Accession #P04202).
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 Mouse T cells. The ED50 for this effect is 0.054-0.214 ng/mL, corresponding to a specific activity of 4.7×106~1.85×107 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
蛋白复溶计算器
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
背景信息
Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factorsand plays a prominent role in a variety of cellular processes, including cell-cycle progression, celldifferentiation,reproductivefunction,development,motility,adhesion,neuronalgrowth,bonemorphogenesis, wound healing, and immune surveillance. TGF- β 1, TGF- β 2 and TGF- β 3 signal via the sameheteromeric receptor complex, consisting of a ligand binding TGF- β receptor type II (T β R-II), and a TGF- βreceptor type I (T β R-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF- βexpression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney,gut, liver, eye, ear, skin, and the nervous system.
别名
TGF-beta1;Tgfb;Tgfb-1;TGFbeta1;CED;DPD1;LAP;TGFB;TGFbeta;TGFB1
