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Recombinant Mouse TNFRSF6/FAS/CD95 Protein (RP00737)

Recombinant Mouse TNFRSF6/CD95/FAS Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP00737
促销价:   ¥760
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
17.4 kDa
Observed MW
25-35 kDa
标签
C-6×His
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.Contact us for customized product form or formulation.
描述
Recombinant Mouse TNFRSF6/FAS/CD95 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Gln22-Arg169) of Mouse TNFRSF6/FAS/CD95 (Accession #P25446 ) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Mouse Apoptosis-mediating surface antigen FAS (Fas) belongs to the death receptor subfamily of the TNFreceptor superfamily and is designated TNFRSF6. Mouse Fas contains 1 death domain and 3 TNFR-Cys repeats.It detected in various tissues including thymus, liver, lung, heart, and adult ovary. As a receptor forTNFSF6/FASLG, The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequentcascade of caspases mediating apoptosis. FAS-mediated apoptosis may have a role in the induction ofperipheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

基因ID
Swiss Prot
别名
Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediatingsurface antigen FAS; FASLG receptor; CD95;Fas;TNFRSF6
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