Recombinant Rhesus macaque TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val77-Leu233) of mouse M-CSF/CSF-1 (Accession #P79337) fused with a 8×His tag at the C-terminus.
TNF-alpha,Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, activating the tumor necrosis factor-mediated (TNF) signaling pathway. The TNF signaling pathway acts as a master regulator of the immune system, which either 1 promotes cell survival and inflammation by activating the canonical NF-kappa-B pathway or 2 trigger programmed cell death (apoptosis and necroptosis). Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation: acts by up-regulating the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line .Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance .Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.Promotes osteoclastogenesis and therefore mediates bone resorption.
