Recombinant Human IGF-I Protein (RP00996)

Recombinant Human IGF-I Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 41 kDa.

Recombinant Human IGF1 enhances p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells.0.01-1ng/mL of Recombinant Human IGF1 can effectively enhance p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.

Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250ng/mL.

Recombinant Human IGF1 promotes the proliferation of MCF-7 cells. The ED₅₀ for this effect is typically 7.5-30 ng/mL.

All(4)|

货号: RP00996

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

34.44 kDa

Observed MW

41 kDa

标签

C-hFc&His

纯度

> 87% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IGF-I Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49 - Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/mL. 3.Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is typically 7.5-30 ng/mL, corresponding to a specific activity of 3.33×10⁴-1.33×10⁵units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。