Recombinant Human FcRn/FCGRT&B2M Protein (RP01037)

Recombinant Human FcRn Protein were determined by SDS-PAGE with Coomassie Blue, showing bands at 38&14 kDa.

Immobilized Human FCGRT&B2M at 5μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linear range of 1-6μg/mL.

Immobilized Trastuzumab on COOH Chip, can bind Human FCGRT&B2M Heterodimer Protein with an affinity constant of 0.22 μM as determined in a SPR assay (OpenSPR).

All(3)|

货号: RP01037

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

30.38 kDa(FCGRT), 11.73 kDa(B2M)

Observed MW

38 kDa(FCGRT), 14(B2M) kDa

标签

C-His(FCGRT)&No tag(B2M)

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human FcRn/FCGRT&B2M Protein is produced by HEK293 cells expression system. The target heterodimer proteins are co-expressed with the sequence (Ala24-Ser297) of human FCGRT (Accession #NP_004098.1) fused with a 6×His tag at the C-terminus and the sequence (Ile21-Met119) of human B2M (Accession #NP_004039.1).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Human FCGRT&B2M at 5 μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linear range of 1-6μg/mL.
2.Immobilized Trastuzumab on COOH Chip can bind Human FCGRT&B2M Heterodimer Protein with an affinity constant of 0.22 μM as determined in a SPR assay (OpenSPR).

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of MHC class I molecules, MHC class I molecules have α1, α2, and α3 proteins which are present on all nucleated cells (excludes red blood cells) and B2M involved in the presentation of peptide antigens to the immune system.

基因ID

2217
567

Swiss Prot

P55899
P61769

别名

FCRN

研究领域