Recombinant Human IL-2 Protein (RP01039)

Recombinant Human IL-2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Measured in a cell proliferation assay using CTLL‑2 mouse T cells. The ED₅₀ for this effect is 0.255‑1.02 ng/mL,corresponding to a specific activity of 9.80×10⁵~3.92×10⁶units/mg.

Recombinant Human IL2 stimulates cell proliferation of the HT-2 Mouse T cells. The ED₅₀ for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 10⁶~4.0× 10⁶ units/mg.

Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.

All(4)|

货号: RP01039

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

16.26 kDa

Observed MW

16 kDa, 18 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Thr153) of human IL2 (Accession #NP_000577.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured in a cell proliferation assay using CTLL‑2 mouse T cells. The ED₅₀ for this effect is 0.255‑1.02 ng/mL,corresponding to a specific activity of 9.80×10⁵~3.92×10⁶units/mg.
2.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED₅₀ for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 10⁶~4.0× 10⁶ units/mg.
3.Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (3)

折叠内容

北京生命科学研究所Oct 232024

评分
Experiment Type: Cell-based assay
Sample: Memory T cell
Description: Human Memory T cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), IL-7 (10 ng/mL, Cat. RP01040) and IL-15 (10 ng/mL, Cat. RP01236), and the cells were observed at 0h, 24h, 36h, 48h. T Cell growth was good and cell proliferation was obvious.

复旦大学附属中山医院Oct 232024

评分
Experiment Type: Cell-based assay
Sample: primary B cell
Description: Human primary B cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), BAFF (10 ng/mL, Cat. RP00018), IL-21 (20 ng/mL, Cat. RP02508), and the cells grew well and formed into clusters after activation.

南京中医药大学Oct 232024

评分
Experiment Type: Cell-based assay
Sample: NK92
Description: After NK92 cells were stimulated with 100 ng/mL IL-2 for 48 h, the number of clusters increased significantly and the activity was enhanced.

文献引用 (1)

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Species

Publishing research using RP01039? Please let us know so that we can cite the reference in this datasheet.

背景信息

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

基因ID

3558

Swiss Prot

P60568

别名

IL2;IL-2;TCGF;lymphokine

研究领域