Recombinant Human IL-2 Protein (RP01039)

Active Recombinant Human IL-2 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 16 kDa and 18 kDa.

Recombinant Human IL2 stimulates cell proliferation of the HT-2 Mouse T cells. The ED₅₀ for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 10⁶~4.0× 10⁶ units/mg.

Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.

All(3)|

货号: RP01039

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

16.26 kDa

Observed MW

16 kDa, 18 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Thr153) of human IL2 (Accession #NP_000577.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED₅₀ for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 10⁶~4.0× 10⁶ units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (3)

折叠内容

CustomerOct 232024

评分
Experiment Type: Cell-based assay
Sample: Memory T cell
Description: Human Memory T cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), IL-7 (10 ng/mL, Cat. RP01040) and IL-15 (10 ng/mL, Cat. RP01236), and the cells were observed at 0h, 24h, 36h, 48h. T Cell growth was good and cell proliferation was obvious.

CustomerOct 232024

评分
Experiment Type: Cell-based assay
Sample: primary B cell
Description: Human primary B cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), BAFF (10 ng/mL, Cat. RP00018), IL-21 (20 ng/mL, Cat. RP02508), and the cells grew well and formed into clusters after activation.

CustomerOct 232024

评分
Experiment Type: Cell-based assay
Sample: NK92
Description: After NK92 cells were stimulated with 100 ng/mL IL-2 for 48 h, the number of clusters increased significantly and the activity was enhanced.

文献引用 (1)

折叠内容

Publishing research using RP01039? Please let us know so that we can cite the reference in this datasheet.

背景信息

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

基因ID

3558

Swiss Prot

P60568

别名

IL2;IL-2;TCGF;lymphokine

研究领域