Recombinant Human FGF-10 Protein (RP01140)

Recombinant Human FGF-10 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-25 kDa.

Recombinant Human FGF-10 Protein promotes proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED₅₀ for this effect is 2.08-8.32 ng/mL, corresponding to a specific activity of 1.20×10⁵~4.81×10⁵ units/mg.

All(2)|

货号: RP01140

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

19.32 kDa

Observed MW

20-25 kDa

标签

No tag

纯度

> 98 % by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS pH7.4.

描述

Recombinant Human FGF-10 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gln38-Ser208) of human FGF10 (Accession #NP_004456.1).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED₅₀ for this effect is 2.08-8.32 ng/mL, corresponding to a specific activity of 1.20×10⁵~4.81×10⁵ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (1)

折叠内容

CustomerOct 232024

评分
Experiment Type: Cell-based assay
Sample: tumor tissue
Description: Patient tissue-derived kidney tumor cells were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071 + 20 ng/mL FGF-10, Cat. RP01140 + 50 ng/mL EGF, Cat. RP01030), and then kidney tumor organoids were formed.

背景信息

This protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.

基因ID

2255

Swiss Prot

O15520

别名

Fibroblast growth factor 10; FGF-10; Keratinocyte growth factor 2;FGF10;KGF2

研究领域