产品中心 / 产品类型 / 重组蛋白 / 白介素

Recombinant Human IL-12A Protein (RP01154)

说明书 联系我们 收藏

Recombinant Human IL-12A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa..

The purity of human IL12A/NKSF1 Protein (Cat.RP01154) was greater than 95% as determined by SEC-HPLC.

Recombinant Human IL12A promotes the proliferation of anti-CD3 antibody activated Jurkat Human acute T lymphocyte leukemia cells. The ED50 for this effect is typically 0.075-0.3 ng/mL.

All(3)|
货号: RP01154
促销价：   ￥780
货    期：现货产品
抗体定制服务咨询

文献引用 (0)

详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human IL-12A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_000873.2) fused with a Flag tag at the C-terminus.
标签
C-Flag
纯度
> 82% by SDS-PAGE.
内毒素
Please contact us for more information.
生物活性
Measured in a cell proliferation assay using anti-CD3 antibody activated Jurkat Human acute T lymphocyte leukemia cells. The ED50 for this effect is typically 0.075-0.3 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

背景信息

Interleukin-12 subunit alpha (IL12A/IL-12p35) is also known as Cytotoxic lymphocyte maturation factor 35 kDa subunit, cytotoxic lymphocyte maturation factor 1, p35, NK cell stimulatory factor chain 1, and interleukin-12 alpha chain. IL12A/IL-12p35 is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

基因ID
3592
Swiss Prot
P29459
别名
P35; CLMF; NFSK; NKSF1; IL-12A;IL12A
研究领域
>