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活性验证

Recombinant Mouse CSF-2/GM-CSF Protein (RP01206)

文献引用 (2) 评论 (4) 说明书 收藏

Active Recombinant Mouse CSF-2/GM-CSF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-35 kDa.

The purity of Mouse GM-CSF/CSF2 Protein (Cat.RP01206) was greater than 90% as determined by SEC-HPLC.

Recombinant Mouse GM-CSF promotes the proliferation of FDC-P1 cells. The ED50 for this effect is typically 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×106~2.5×107 units/mg.

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货号: RP01206

促销价：   ￥560
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
14.95 kDa
Observed MW
20-35 kDa
标签
N-His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Mouse CSF-2/GM-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Lys141) of mouse GM-CSF/CSF2 (Accession #NP_034099.2) fused with a 6×His tag at the N-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×106~2.5×107 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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客户数据及评论 (4)

折叠内容
CustomerNov 202024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
After three days of stimulation of mouse bone marrow cells with 40 ng/ml GM-CSF (Cat. RP01206), the purity of myelogenic inhibitory cells induced by flow cytometry was 82.98%.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
In vitro induction culture of mouse bone marrow derived dendritic cells (BMDC): C57BL/6 mouse bone marrow cells from femur and tibia were induced with IL-4 (10 ng/mL, Cat. RP01161) and GM-CSF (20 ng/mL, Cat. RP01206) to differentiate into BMDC. DCs were labeled with CD11c, and DCs maturation was detected by CD80 and CD86 at 7 days after induction. Flow cytometry result showed that the proportion of cells expressed CD11c was as high as 89.4%, and more than half of the cells expressed CD80 and CD86, indicating BMDC was successfully induced.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
Mouse alveolar macrophages
Description
Mouse alveolar macrophages (AMs) were stimulated with recombinant mouse GM-CSF (40 ng/mL, Cat. RP01206) for 30 days. The proliferation effect was good with fewer polarized cells.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
In vitro induction culture of mouse bone marrow derived dendritic cells (BMDC): mouse bone marrow monocytes were treated with GM-CSF (20 ng/mL, Cat. RP01206) and IL-4 (10 ng/mL, Cat. RP01161) to differentiated into BMDC. The expression of CD11c and MHC Ⅱ were detected by flow cytometry.

文献引用 (2)

折叠内容

Publishing research using RP01206? Please let us know so that we can cite the reference in this datasheet.

    背景信息

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also known as Colony stimulating factor 2 (granulocyte-macrophage), is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells, and is secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exitthe circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. The active form of the protein is found extracellularly as a homodimer. Human GM-CSF glycosylated in its mature form. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising. GM-CSF is also used as a medication to stimulate the production of white blood cells following chemotherapy.

    基因ID
    12981
    Swiss Prot
    P01587
    别名
    GMCSF;CSF2
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