Recombinant Rat MMP-9 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions, showing single bands at 90-100 kDa and 180-200 kDa, respectively.
Recombinant Rat MMP9 induces cell migration of the Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration.
Lyophilized from a 0.22 μm filtered solution of 50mM Tris,10mM CaCl2,150mM NaCl, pH 7.5.Contact us for customized product form or formulation.
描述
Recombinant Rat MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu35-Pro708) of rat MMP-9 (Accession #NP_038627.1.) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
1.Measured in a cell migration assay using Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration. 2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1600 pmoles/min/μg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。