Recombinant Human IL-12/IL-12A&IL-12B Protein (RP01232)

Active Recombinant Human IL-12A&IL-12B Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 37 kDa and 40-55 kDa.

Immobilized Recombinant Human IL-12 at 1μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.

Recombinant Human IL-12 promotes the proliferation of anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED₅₀ for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×10⁶-1.0×10⁷units/mg.

Recombinant Human IL-12/IL-12A&IL-12B induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is 2-8 ng/mL, corresponding to a specific activity of 1.25×10⁵~5×10⁵ units/mg.

All(4)|

货号: RP01232

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

25.54 kDa(IL-12A), 35.54 kDa(IL-12B)

Observed MW

37 kDa(IL-12A), 40-55 kDa(IL-12B)

标签

C-His(IL12B)&C-Flag(IL12A)

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Active Recombinant Human IL-12A&IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg23-Ser219) of human IL-12 (Accession #NP_002178.2/NP_000873.2) fused with a 6×His tag at the C-terminus(IL12B),Flag tag at the C-terminus(IL12A).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL-12 at 1 μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.
2.Measured in a cell proliferation assay using anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED₅₀ for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×10⁶-1.0×10⁷units/mg.
3.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is 2-8 ng/mL, corresponding to a specific activity of 1.25×10⁵~5×10⁵ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。