产品中心 / 产品类型 / 重组蛋白 / SARS-CoV-2相关蛋白
| 种属 | SARS-CoV-2 |
|---|---|
| 表达宿主 | HEK293 cells |
| 描述 | Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus. |
| 标签 | C-His |
| 纯度 | >95% by SDS-PAGE;> 95% by HPLC |
| 内毒素 | < 0.1 EU/μg of the protein by LAL method. |
| 生物活性 | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike RBD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. 2.Immobilized human ACE2 on COOH Chip can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR). |
| 制剂 | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation. |
| 储存 | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
| 复溶 | For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| 基因ID | 43740568 |
|---|---|
| 别名 | Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein |
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