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活性验证

Recombinant SARS-CoV-2 Spike RBD Protein (RP01258)

文献引用 (8) 说明书 收藏

Recombinant SARS-CoV-2 Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.

The purity of SARS-COV-2 Spike RBD Protein with His tag (Cat.RP01258) was greater than 95% as determined by SEC-HPLC.

Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay (Nicoya OpenSPR).

Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-2.96 ng/mL.

Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-1.69 ng/mL.

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货号: RP01258
促销价：   ￥1600
货    期：现货产品
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详细信息

种属
SARS-CoV-2
表达宿主
HEK293 cells
Calculated MW
25.94 kDa
Observed MW
35-40 kDa
标签
C-His
纯度
>95% by SDS-PAGE;> 95% by HPLC
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-2.96 ng/mL.
2.Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay (Nicoya OpenSPR).
3.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD (Catalog: RP01258) at 2 μg/mL (100 μL/well) can bind Human ACE-2 (Catalog: RP01275) with a linear range of 0.001-1.69 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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文献引用 (8)

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Publishing research using RP01258? Please let us know so that we can cite the reference in this datasheet.

背景信息

基因ID
43740568
别名
Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein
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