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|描述||Recombinant SARS-CoV-2(2019-nCoV) Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.|
|纯度||>95% by SDS-PAGE;> 95% by HPLC|
|内毒素||< 0.1 EU/μg of the protein by LAL method.|
|生物活性||1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike RBD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL.|
2.Immobilized human ACE2 on COOH Chip can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR).
|制剂||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.|
|储存||Store the lyophilized protein at -20°C to -80 °C for long term.|
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
or This product is stable at ≤ -70°C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
|复溶||For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
|别名||Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein|
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