Recombinant SARS-CoV-2 Envelope Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 12 kDa.
Immobilized Recombinant SARS-CoV-2 Envelope at 2μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid with a linear range of 1.2-41.1 ng/mL.
Supplied as a 0.22 μm filtered solution in 20mM Tris,250mM NaCl,0.5%TritonX-100,pH 8.0.Contact us for customized product form or formulation.
描述
Recombinant SARS-CoV-2(2019-nCoV) Envelope Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Val75) of SARS-COV-2(2019-nCoV) Envelope (Accession #QHD43418.1) fused with an initial Met,a 6×His,Avi tag at the N-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Envelope at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid with a linear range of 1.2-41.1 ng/mL.
SARS-CoV-2 E protein induces Toll like receptor 2-mediated neonatal lung injury in a model of COVID-19 viremia that is rescued by the glucocorticoid ciclesonide
Journal
American journal of physiology. Lung cellular and molecular physiology
Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers