Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(D614G) Protein (RP01291LQ)

Recombinant SARS-COV-2 S1+S2 ECD（S-ECD）(D614G) Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 180-200 kDa.

Immobilized recombinant SARS-CoV-2 Spike S1+S2 ECD at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-2.58 ng/mL.

All(2)|

货号: RP01291LQ

详细信息

种属

SARS-CoV-2

表达宿主

HEK293 cells

Calculated MW

133.52 kDa

Observed MW

180-200 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

描述

Recombinant SARS-CoV-2 S1+S2 ECD（S-ECD）(D614G) Protein with His tag is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208(Asp614Gly)) of sars-cov-2 S1+S2 ECD（S-ECD）(D614G) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized recombinant SARS-CoV-2 Spike S1+S2 ECD at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-2.58 ng/mL.

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

基因ID

43740568

Swiss Prot

P0DTC2

别名

Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein