Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(D614G) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208(Asp614Gly)) of sars-cov-2 S1+S2 ECD(S-ECD)(D614G) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1+S2 ECD at 2 μg/mL (100μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.55 ng/mL.
制剂
Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
储存
This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。