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Recombinant Rat TNF-alpha Protein (RP01322)

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Recombinant Rat TNF-alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18kDa.

Recombinant Rat TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.0125-0.05 pg/mL.

All(2)|
货号: RP01322
促销价：   ￥780
货    期：现货产品
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详细信息

种属
Rat
表达宿主
HEK293 cells
描述
Recombinant Rat TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu80-Leu235) of Rat TNFA (Accession #NP_036807.1) fused with an 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.0125-0.05 pg/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Tumor necrosis factor alpha is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin.TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

基因ID
24835
Swiss Prot
P16599
别名
DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFα;TNFA
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