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活性验证

Recombinant Mouse IL-1 beta Protein (RP01340)

文献引用 (5) 评论 (1) 说明书 收藏

Recombinant Mouse IL-1 beta Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at showing bands at 19-20 kDa..

Immobilized Mouse IL1B at 10 μg/mL (100 μL/well) can bind Human IL1R2 with a linear range of 0.03125-0.8036 μg/mL.

Recombinant Mouse IL-1B promotes the proliferation of D10.G4.1 mouse helper T cells. The ED50 for this effect is 3.10-12.40 pg/mL, corresponding to a specific activity of 8.06×107~3.23×108 units/mg..

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货号: RP01340

促销价：   ￥1100
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
E. coli
Calculated MW
18.23 kDa
Observed MW
19-20 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val118-Ser269) of mouse IL-1 beta (Accession #NP_032387.1) fused with 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Mouse IL1B at 10 μg/mL (100 μL/well) can bind Human IL1R2 with a linear range of 0.03125-0.8036 μg/mL.
2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 42.1-168.4 pg/mL, corresponding to a specific activity of 5.94×106-2.37×107units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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客户数据及评论 (1)

折叠内容
CustomerNov 202024
评分
Experiment Type
Cell-based assay
Sample
C2C12
Description
The cytokine combination (IFN-γ:149.2ng/ml, Cat.RP01070; TNF-α:200ng/ml, Cat.RP01071; IL-1β : 2.7ng/ml Cat.RP01340) was dissolved into 10X cytomix using PBS. Then use the gradient dilution of 10 X cytomix stimulate C2C12 cells for 24 h, concentration as follows (0.125 X, 0.25 X, 0.5 X, 1 X, 1.25 X), induction of cell damage, finally determined by MTT were used to detect cell damage

文献引用 (5)

折叠内容

Publishing research using RP01340? Please let us know so that we can cite the reference in this datasheet.

背景信息

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.

基因ID
16176
Swiss Prot
P10749
别名
IL-1 beta;IL-; Il-1b; IL-1beta;Il1b
研究领域
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