| 种属 | Mouse |
|---|---|
| 表达宿主 | E. coli |
| 描述 | Active Recombinant Mouse IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val118-Ser269) of mouse IL-1 beta (Accession #NP_032387.1) fused with 6×His tag at the C-terminus. |
| 标签 | C-His |
| 纯度 | > 95% by SDS-PAGE. |
| 内毒素 | < 1 EU/μg of the protein by LAL method. |
| 生物活性 | 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse IL1B at 10 μg/mL (100 μL/well) can bind Human IL1R2 with a linear range of 0.03125-0.8036 μg/mL. 2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 42.1-168.4 pg/mL. |
| 制剂 | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| 储存 | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| 复溶 | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
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