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活性验证

Recombinant Mouse IL-3 Protein (RP01386)

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货号: RP01386
价 格：  1000.00
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
19.38 kDa
Observed MW
10-35 kDa
标签
C-His
纯度
≥ 90 % as determined by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述

Recombinant Mouse IL-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Cys166) of mouse IL3 (Accession #NP_034686.2.) fused with  a 6×His tag at the C-terminus.

储存
Store at -20℃ （1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
Measured in a cell proliferation assay using BaF3 mouse pro-B cells. The ED50 for this effect is 1.5-6 pg/mL, corresponding to a specific activity of 1.67 × 108~6.67× 108 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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客户数据及评论 (1)

折叠内容
中国医学科学院血液病医院 (血液学研究所)Nov 122024
评分
Experiment Type
Cell-based assay
Sample
hMPC
Description
Recombinant mouse IL-3 (Cat. RP01386) from ABclonal and Per Brand were used to culture human mesenchymal precursor cells (hMPC) 32F and mouse pro-B cells BAF3. After 2 and 4 days, the effect of different brand proteins were reflected by cell counting. Results showed that the activity of IL-3 from ABclonal and Peprotech were not much different. But the cell number of ABclonal group at 4 days in the left panel was less than that of Peprotech group, which remain to be elucidated.
更多

Publishing research using RP01386? Please let us know so that we can cite the reference in this datasheet.

背景信息

IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

基因ID
16187
Swiss Prot
P01586
别名
BPA;Csfmu;HCGF;IL-3;MCGF;PSF;IL3