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Active Recombinant Human Mature TGF-beta 1 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions, showing bands at 15 kDa and 25-30 kDa, respectively.
Immobilized Human TGF-beta 1 at 2 μg/mL (100 μL/well) can bind Human TGFBR2 with a linear range of 0.78-11 ng/mL.
Recombinant Human Mature TGFB1 inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 42-168 pg/mL,corresponding to a specific activity of 6×106~2.4×107 units/mg.
Active Recombinant Human mature TGF-beta1 induces EMT in A549 cells. As the concentration of TGF-beta1 increased, the expression of epithelial cell marker E-cad significantly downregulated.(Customer feedback data)
Active Recombinant Human mature TGF-beta1 stimulates smad2/3 autophosphorylation in Human Umbilical Vein Endothelial Cells (HUVEC). 10ng/mL of Recombinant Human TGF-beta 1 can effectively enhance smad2/3 autophosphorylation.(Customer feedback data)
Active Recombinant Human Mature TGF-beta 1 induces MCF7 cells EMT with a concentration of 12.5 ng/mL. After 10 h, the cell morphology significantly altered, appearing a fusiform shape (figure 1). After 48 h, the change of EMT associated genes are as figure 2. Results showed that the induction was successful.(Customer feedback data)
Recombinant mouse IL-23(Cat. RP02928LQ) and human TGF-β1 were used to induce the mouse T cells differentiate into Th17 cells. Flow cytometry was used to detect the expression of IL-17A. Results showed that Th17 cells were induced successfully.(Customer Feedback Data)
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TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.
