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活性验证

Recombinant Human Mature TGF-beta 1 Protein (RP01458)

文献引用 (3) 评论 (7) 说明书 收藏

Recombinant Human Mature TGF-beta 1 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions, showing bands at 15 kDa and 25-30 kDa, respectively.

Immobilized Human TGF-beta 1 at 2 μg/mL (100 μL/well) can bind Human TGFBR2 with a linear range of 0.78-11 ng/mL.

Recombinant Human Mature TGF-beta 1 inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 12.85-51.40 pg/mL, corresponding to a specific activity of 1.95×107~7.78×107 units/mg.

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货号: RP01458

促销价：   ￥780
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
12.79 kDa
Observed MW
15 kDa
标签
No tag
纯度
> 95% by SDS-PAGE.
内毒素
<0.1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of 50 mM Glycine,150 mM NaCl,pH3.5.
描述
Recombinant Human Mature TGF-beta 1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala279-Ser390) of human Mature TGF-beta 1 (Accession #NP_000651.3) fused with no tag.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-beta 1 at 2 μg/mL (100 μL/well) can bind Human TGFBR2 with a linear range of 0.78-11 ng/mL.
2.Measured by its ability to inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 12.85-51.40 pg/mL, corresponding to a specific activity of 1.95×107~7.78×107 units/mg.
3.Measured in a cell proliferation assay using Mv.1.lu cells. The ED50 for this effect is 0.08-0.32ng/mL,corresponding to a specific activity of 3.13×106~1.25×107 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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客户数据及评论 (7)

折叠内容
CustomerNov 202024
评分
Experiment Type
Cell-based assay
Sample
LX-2
Description
LX-2 cell line was stimulated with 10 ng/mL TGF-β1 (Cat.RP01458) for 24 h and α-SMA expression was detected by immunofluorescence (green fluorescence is α-SMA staining).
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
A549
Description
Recombinant human TGF-β (Cat. RP01458) was used to treat human lung adenocarcinoma cells A549 for 48 h. Western-blot result showed that the epithelial cell marker E-card was down-regulated, indicating the epithelial–mesenchymal transition was induced successfully.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
HUVEC
Description
Western-blot was used to measure the ability of TGF-β (Cat. RP01458) to stimulate smad2/3 autophosphorylation in human umbilical vein endothelial cells (HUVEC). Result showed that 10 ng/mL of recombinant human TGF-β effectively enhanced smad2/3 autophosphorylation.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
MCF-7
Description
Recombinant human TGF-β (12.5 ng/mL, Cat. RP01458) was used to treat human breast carcinoma cells MCF7 for 10 and 48 h respectively. Cell morphology changed significantly, showing fusiform, and was biased towards mesenchymal cells, indicating the epithelial–mesenchymal transition was induced successfully.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
Beas-2B
Description
Alveolar epithelial cells Beas-2B were stimulated by 10 ng/mL and 20 ng/mL of TGF-β1 (Cat. RP01458) for 12 h respectively to construct in vitro pulmonary fibrosis model. Western-blot result showed that both of the EMT marker N-cad and myofibroblast marker α-SMA were up-regulated.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
T cell
Description
Recombinant mouse IL-23 (Cat. RP02928LQ) and human TGF-β (Cat. RP01458) induced mouse Th17 differentiation. The expression of IL-17α was detected by flow cytometry.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
A549
Description
Human lung adenocarcinoma cells A549 were treated with recombinant human TGF-β (10 ng/mL, Cat. RP01458) from ABclonal and M Brand for 48 h respectively to construct in vitro pulmonary fibrosis model. Western-blot result showed that TGF-β from ABclonal induced successfully, but MCE failed. The same result was obtained in rat hepatic stellate cells Hsc-T6.

文献引用 (3)

折叠内容

Publishing research using RP01458? Please let us know so that we can cite the reference in this datasheet.

    背景信息

    TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.

    基因ID
    7040
    Swiss Prot
    P01137
    别名
    TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; transforming growth factor beta-1;TGF-beta 1;CED;DPD1;LAP;TGFB;TGFbeta;TGF-β
    研究领域
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